A complete pipetting cycle includes steps such as tip installation-volume setting-aspiration and discharge removal of the tip. Each step has operating specifications that need to be followed.

  1. Tip Installation

For a single-channel pipette, the end of the pipette is inserted vertically into the tip, and it can be tightened by gently pressing left and right; for a multi-channel pipette, the first channel of the pipette is aligned with the first tip, and inserted at an angle, shake back and forth slightly to tighten.

Do not hit the pipette repeatedly to ensure the airtightness of the tip. Assembling the tip in this way for a long time will cause the components of the pipette to loosen due to strong impact, and even cause the knob to adjust the scale to get stuck.

  1. Capacity setting

When adjusting from a large volume to a small volume, just turn it counterclockwise to the scale; when adjusting from a small volume to a large volume, you can first adjust the set volume clockwise, and then return to the set volume to ensure the best accuracy.

Do not turn the adjustment knob out of the range, otherwise, the mechanical device inside the pipette will be damaged.

  1. Aspiration and discharge

Aspiration Press the pipette button to the first position, release the button, and start aspiration. Remember not to go too fast, otherwise, the liquid will enter the tip too quickly and cause the liquid to be sucked back into the pipette.

Drain liquid Close to the container wall, first press the first gear, pause for a while, then press the second gear to drain the remaining liquid.

  1. Correct placement of the pipette

After use, it can be hung upright on the pipette rack, but be careful not to drop it. When there is liquid in the pipette tip, do not place the pipette horizontally or upside down, so as to prevent the liquid from flowing back and corroding the piston spring.

If not in use, adjust the volume of the pipette to the maximum scale, so that the spring is in a relaxed state to protect the spring.

Suzhou Yider Biotechnology Co., Ltd. not only has Pipette Tips but also PCR Tube and other products, welcome to check our official website.